Accutase® : Cell Detachment Reagent Applications: For gentle and effective detachment of adherent primary cells and stem cell lines and tissues. Proven effective in detaching fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, stellates, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
Does not need to be washed out or neutralized like trypsin Does not contain mammalian or bacterial-derived products Accutase allows a greater recovery rate of frozen hESCs
Function: Enzyme with proteolytic and collagenolytic activity
Testing: Accutase has been extensively tested in research and biopharmaceutical laboratories.